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Understanding Quality Control in Single-Cell RNA Sequencing: Part IV – Detecting Ambient RNA

Understanding Quality Control in Single-Cell RNA Sequencing: Part IV – Detecting Ambient RNA

by seb_insigene | Jul 18, 2024 | Blog

In our series on single-cell RNA sequencing (scRNA-seq) quality control (QC), we have covered detecting low UMI cells, empty droplets, and doublets. In this fourth part, we will focus on detecting ambient RNA, a common source of contamination in scRNA-seq data. We...
Understanding Quality Control in Single-Cell RNA Sequencing: Part III – Detecting Doublets

Understanding Quality Control in Single-Cell RNA Sequencing: Part III – Detecting Doublets

by seb_insigene | Jul 11, 2024 | Blog

In the previous parts of our series on single-cell RNA sequencing (scRNA-seq) quality control (QC), we discussed detecting low UMI cells and empty droplets. In this third part, we will focus on detecting doublets. Doublets occur when two or more cells are captured in...
Understanding Quality Control in Single-Cell RNA Sequencing: Part II – Detecting Empty Droplets

Understanding Quality Control in Single-Cell RNA Sequencing: Part II – Detecting Empty Droplets

by seb_insigene | Jul 4, 2024 | Blog

In our first blog post, we discussed the importance of detecting and filtering out low UMI cells to ensure high-quality single-cell RNA sequencing (scRNA-seq) data. In this second part of our series on scRNA-seq quality control (QC), we will focus on detecting empty...
Understanding Quality Control in Single-Cell RNA Sequencing: Part I – Detecting Low UMI Cells

Understanding Quality Control in Single-Cell RNA Sequencing: Part I – Detecting Low UMI Cells

by seb_insigene | Jun 28, 2024 | Blog

Single-cell RNA sequencing (scRNA-seq) has revolutionized our ability to explore cellular heterogeneity, providing insights into the complexity of gene expression at an unprecedented resolution. However, the accuracy of the conclusions drawn from scRNA-seq data...

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